Influence of Aggregate Composition and Dispersion on its Cementation During Carbonate Biomineralization

The results of the study of carbonate biomineralization by ureolytic bacterial cultures in a aggregate medium of different composition and dispersion as a result of percolation cementation method are presented. Quartz sand, marble and cement stone were considered as aggregates, which were crushed and fractionated to three fractions: 1.25–0.63; 0.63–0.315; 0.315–0.16. Physical and chemical factors determining the intensity of induction processes have been established. The microstructure of the cemented layers is analyzed, the features of new formations are considered. The time limits of the consolidated layer formation within two fractions (0.63–0.315 and 0.315–0.16) were revealed, which directly depend on the percolation depth of the solution with precursors and bacterial inoculum. It has been shown that the bacterial culture of Lysinibacillus sphaericus exhibits active cementing properties to the greatest extent. The maximal thickness of the aggregate layer consolidated by calcium carbonate crystals was formed in samples with carbonized cement stone with the aggregate fraction of 0.63–0.315 mm.

V.V. STROKOVA¹, Doctor of Sciences (Engineering) (This email address is being protected from spambots. You need JavaScript enabled to view it.),
U.N. DUKHANINA¹, Engineer,
D.A. BALITSKY¹, Engineer,
O.I. DROZDOV¹, Master degree student,
V.V. NELUBOVA¹, Doctor of Sciences (Engineering);
O.V. FRANK-KAMENETSKAYA², Doctor Sciences (Geology and Mineralogy),
D.Yu. VLASOV², Doctor Sciences (Biology)

¹ Belgorod State Technological University named after V.G. Shukhov (46, Kostyukova Street, Belgorod, 308012, Russian Federation)
² Saint Petersburg University (7/9, University Embankment, St. Petersburg, 199034, Russian Federation)

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